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The retention time may be the length of time it will require for the component to maneuver from your injector to the detector.By lowering the pH in the solvent in a very cation exchange column, For illustration, a lot more hydrogen ions are offered to compete for positions about the anionic stationary section, thereby eluting weakly certain cations
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Stationary section chemistry dictates the affinity in the sample components to stick or keep over the column since the mobile stage moves the sample in the column. Consequently, the sample factors traverse the column and elute at different premiums.In summary, choosing the proper HPLC column is critical to obtaining prosperous molecular separations
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